Composite

Part:BBa_K2542012:Design

Designed by: Yaoqiang Zheng   Group: iGEM18_NEFU_China   (2018-10-05)


pCYC-Stemloop1-EGFP-tCYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

We assembled it ourselves. We obtained the promoter and terminator from the plasmid. The sequence of Stemloop was obtained from the literature. Then we assembled and got the final plasmid.

References